Canine and Feline Blind Bronchoalveolar Lavage (BAL)

Common indications for blind BAL:

– Chronic cough
– Chronic increase in respiratory effort or tachypnoea
– Diffuse changes in lung fields on imaging, if there are associated clinical signs

Potential risks of the procedure:

– Poor anaesthetic stability
– Bronchospasm
– Pneumothorax

*NB these risks are inherent, associated more with the underlying disease process than the procedure
itself.

Equipment:

  • Soft feeding tube/nasogastric feeding tube (without stilette)
  • 2-3 x 5ml syringe (small dogs and cats) or 2-3 x20ml syringe (large dogs), 1x 1ml syringe
  • Warm, sterile saline
  • Needle
  • Universal container
  • 1-2 EDTA tubes
  • 2-3 microscope slides
  • Scalpel blade
  • Sterile endotracheal tube
  • Short non-sprung mouth gag
  • Sterile gloves
    *Optional- double swivel tracheal tube connector with capped port
  • Anaesthetic monitoring including SpO2

Method:

The patient should be anaesthetised, intubated with sterile endotracheal tube, placed in sternal or lateral recumbency (see below). 

 Put on the sterile gloves.

 Measure the tube to the end of the rib cage to ensure it is long enough.

 If the feeding tube distal ports are to the side, use the scalpel blade to cut the end of the tube off in a sterile manner to make it ‘end tip’ rather than ‘side tip’.

 Fill appropriate syringes with warm sterile saline.

 Ensure the patient is stable and asleep, with a high SpO2 reading (manual ventilate for a breath or two if needed).

 Turn off the gaseous anaesthesia, squeeze the bag to empty it of the gas and disconnect the from the ET tube (* or use the capped port and do not need to disconnect).

 Insert the sampling tube through the sterile ET tube and advance it until it will go no further
(presumably lodged in a bronchus).

 Connect the saline filled syringe, rapidly instil the fluid, and immediately aspirate while an assistant performs gentle coupage.

 If there is negative pressure, withdraw the tube slightly and re-aspirate. If the tube fills with air,
move the tube slightly, detach, expel the air and try again.

 It is unusual to recover all of the fluid instilled. Remaining saline will be resorbed by the patient
without complication.

 Remove the tube with the syringe still attached and ensure the tip is facing up on removal to prevent loss of fluid.

 Insert the tip of the tube in to the plain universal container, expel the fluid from the syringe through the tube (flushing it) and then remove the syringe (keep tube in the universal container), fill it with air and once again attach to the tube and expel the air through the tube to remove any remaining fluid.

 Repeat the procedure one more time collecting the sample in to the same universal container.

 Once both samples have been collected use the 1ml syringe to remove a representative sample to place in to the EDTA container for cytology.

 The last drops of the fluid from this syringe can be used to make 1-3 slides for microscopy, by lightly smearing a drop of fluid with another microscope slide and allowing this to dry.

 Submit the samples for cytology and culture. PCR testing is often helpful where there is suspicion for an infectious cause (based on clinical findings, or subsequent to cytological description).

Tips:

  • Some clinicians like to pre-medicate the patient with inhaled or parenteral bronchodilators
    to reduce the risk of bronchospasm.
  •  If one side of the pulmonary field appears to have more marked changes, place the patient
    in lateral recumbancy with this side lowermost, as there is a chance that gravity will take the
    tube to this side.
  •  Gold standard is often considered to BAL from 2 sites (eg left and right lung fields). However
    this may not alter diagnosis and the increased cost and minor increase in risks should be
    considered.
  • Warming the flush fluid decreases the chance of bronchospasm and helps to increase cell
    yield
  • A frothy sample suggests the alveolar space has been sampled (froth = presence of
    surfactant). Turbid, flocculent samples are often diagnostic. If yield appears to be poor in
    quantity or quality, it can be increased by performing the flush 2-3 times in the same area
  • Total maximum 2ml/kg saline flush is recommended, split this into 2-3 aliquots.
    This is important for cats and small dogs. Large dogs rarely require as much as 2ml/kg saline
    to provide sufficient sample.
  • Poor yield may reflect the presence of thick discharge which is difficult to aspirate. Larger
    bore sampling tubes may be worth a try: they will not reach as far into the bronchial tree,
    but, they will be more successful in collecting thick, viscous material.
  • Very thick mucus in the sample may “trap” diagnostic cells and remove them from
    cytological analysis. It can help to make a smear from a small amount of mucopurulent
    material in such a case.
  • A light plane of anaesthesia is often preferred as this is less likely to suppress coughing. A
    little coughing can aid fluid and cellular yield. A short mouth gag without a spring is safe and
    protects the ET tube.
  • If a connector with a capped port is unavailable, flow-by oxygen should be provided during
    the procedure if it becomes more protracted than expected. Increments of intravenous
    anaesthetic can be administered, (or total intravenous anaesthesia used), but note that BAL
    is typically a rapid procedure; a duration sufficient to cause hypoxia would be unusual.

In case of complication:

  • Both bronchospasm and pneumothorax will present in a similar manner, with hypoxia,
    respiratory compromise and resistance to intermittent positive pressure ventilation.
    Bronchospasm typically occurs at the time of BAL, pneumothorax may occur immediately or
    develop more slowly.
  • Intravenous terbutaline (*NB off-licence) can be used to reverse bronchospasm (expect
    tachycardia).
  • Thoracic radiography can be undertaken to detect pneumothorax but diagnostic
    thoracocentesis is quicker, and can progress easily to a therapeutic procedure.
  • Typically pneumothorax can be successfully treated with a single thoracocentesis only.
    Access to a chest drain (eg MILA – simple to place), would be advisable as a precaution
    NB Complications of BAL are rare and in the vast majority of cases, can be treated successfully and
    with ease. Just be prepared!

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